Amount per vial
Insulin and insulin-like growth factor 1 (IGF1) are two closely related proteins and share similarities in both primary and tertiary structure. The homology is paralleled by similarities in the structures of their receptors, the insulin receptor (InsR) and the IGF1 receptor (IGF1R), which belong to the same family of receptor tyrosine kinases.
When studying the InsR and IGF1R is it therefore important to use specific antibodies that does not cross-react with both receptors. NNC0124-0226 is a highly specific IGF1R monoclonal antibody that lack cross-reactivity to the InsR. NNC00124-0226 is an IgG4 monoclonal antibody directed against alfa-subunit of the IGF1R.
Also, unlike earlier monoclonal antibodies reported in the literature, this antibody shows cross-species reactivity to the extracellular domains of mouse, rat, pig, and human receptors, indicating that it binds conserved epitopes. Furthermore, the antibodies work well in several different assay formats and therefore provide a new tool to study InsR and IGF1R biology with translation across several species and experimental model systems. NNC0124-0226 differ in one amino acid compared to the mAb 227 in the reference by Ørstrup et al 2019 listed in the reference section and therefore displays the same specificity as mAb 227.
Amount per vial
The antibodies are of very high purity and have a minimal amount of aggregates as determined by SEC-HPLC. The isotype control (NNC9416-0001) is anti-trinitrophenol (aTNP), the isotype control standard used in most in vitro and in vivo applications since the antigen is not considered present in mammals.
||I sotype control (NNC9416-0001)|
|Production host||HEK cells||CHO cells|
|Antigen||Human IGF1 receptor||
|Original species||Human/rabbit (rabbit variable domains)||-|
|Buffer composition||PBS, pH 7.4 with 0.02% Na-azide||
PBS, pH 7.4 with
|Concentration||0.99 mg/mL||0.93 mg/mL|
|Theoretical mass||144349.8 Da||144774.3 Da|
CHO: Chinese hamster ovary; EU: endotoxin unit; HEK: human embryonic kidney; PBS: phosphate buffered saline; SEC: size exclusion chromatography
NNC0124-0226 displays cross-species reactivity to the extracellular domains of mouse, rat, pig, and human insulin receptors (other species not tested), indicating that it binds conserved epitopes. Furthermore, the antibody works well in several different assay formats, see the table.
|WB (only non-reduced)||+|
|ELISA (tested as capture Ab in sandwich ELISA)||+++|
|Immunohistochemistry on frozen section||Not tested|
|Human, mouse, rat, pig (other species not tested)|