Amount per vial
Insulin and insulin-like growth factor 1 (IGF1) are two closely related proteins and share similarities in both primary and tertiary structure. The homology is paralleled by similarities in the structures of their receptors, the insulin receptor (InsR) and the IGF1 receptor (IGF1R), which belong to the same family of receptor tyrosine kinases.
When studying the InsR and IGF1R is it therefore important to use specific antibodies that does not cross-react with both receptors. NNC0276-3000 is a highly specific InsR monoclonal antibody that lack cross-reactivity to the IGF1R. NNC0276-3000 is a human IgG1 monoclonal antibody directed against alfa-subunit of insulin receptor.
Also, unlike earlier monoclonal antibodies reported in the literature, this antibody shows cross-species reactivity to the extracellular domains of mouse, rat, pig, and human receptors, indicating that it binds conserved epitopes. Furthermore, the antibodies work well in several different assay formats and therefore provide a new tool to study InsR and IGF1R biology with translation across several species and experimental model systems.
Amount per vial
The antibodies are of very high purity and have a minimal amount of aggregates as determined by SEC-HPLC. The isotype control (NNC9416-0015) is anti-trinitrophenol (aTNP), the isotype control standard used in most in vitro and i n vivo applications since the antigen is not considered present in mammals.
|Property||NNC0276-3000||Isotype control (NNC9416-0015)|
|Production host||HEK cells||CHO cells|
|Antigen||Human insulin receptor||
|Buffer composition||PBS, pH7.4 with 0.02% Na-azide||
PBS, pH7.4 with
|Concentration||0.98 mg/mL||1.05 mg/mL|
|Theoretical mass||144078.1 Da||145493.6 Da|
|Endotoxin||<0.06 EU/mg||<0.06 EU/mg|
CHO: Chinese hamster ovary; EU: endotoxin unit; HEK: human embryonic kidney; PBS: phosphate buffered saline; SEC: size exclusion chromatography.
NNC0276-3000 displays cross-species reactivity to the extracellular domains of mouse, rat, pig, and human insulin receptors (other species not tested), indicating that it binds conserved epitopes. Furthermore, the antibody works well in several different assay formats, see the table below.
|WB (reduced and non-reduced)||+|
|ELISA (tested as capture Ab in sandwich ELISA)||+++|
|Immunohistrochemistry on frozen section||Not tested|
Human, mouse, rat, pig (other species not tested)